Enzymatic biosensor for the electrochemical detection of 2,4-dinitrotoluene derivatives.

MARCELA C. RODRIGUEZ; MARIELA R. MONTI; CARLOS E. ARGARAÑA; GUSTAVO A. RIVAS.

TALANTA

Elsevier

Lugar: Amsterdam; Año: 2006 vol. 68 p. 1671 - 1671

0039-9140

p style="TEXT-ALIGN: justify">In this work, we demonstrate for the first time that 4-methyl-5-nitrocatechol (4M5NC) and 2,4,5-trihydroxytoluene (2,4,5-THT), two compounds obtained from the 2,4-DNT biodegradation are recognized by polyphenol oxidase as substrates. An amperometric biosensor is described for detecting these compounds and for evaluating the efficiency of the 2,4-DNT conversion into 4M5NC in the presence of bacteria able to produce the 2,4-DNT-biotransformation. The biosensor format involves the immobilization of polyphenol oxidase into a composite matrix made of glassy carbon microspheres and mineral oil. The biosensor demonstrated to be highly sensitive for the quantification of 4M5NC and 2,4,5-THT. The analytical parameters for 4M5NC are the following: sensitivity of (7.5